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The best method for extracting European bilberry extract.

2024-12-01
Related Product
Bilberry Extract
We are the leading bilberry extract manufacturer and also the leading supplier and exporter of bilberry extract. We specialize in providing natural and organic bilberry extract to meet your needs.
Bilberry Extract

1. Introduction

European bilberry (Vaccinium myrtillus) is a rich source of bioactive compounds such as anthocyanins, flavonoids, and phenolic acids. These compounds have been associated with various health benefits, including antioxidant, anti - inflammatory, and vision - improving properties. Extracting these valuable compounds from European bilberry in an efficient and sustainable manner is of great importance in the fields of pharmaceuticals, nutraceuticals, and food industries. In this article, we will explore in - depth different methods of extraction, namely solvent extraction and supercritical fluid extraction, and analyze their pros and cons in terms of yield, quality, and environmental impact to determine the best extraction method.

2. Solvent Extraction

2.1 Principles

Solvent extraction is a traditional and widely used method for extracting bioactive compounds from plant materials. In the case of European bilberry, the process typically involves soaking the bilberry fruits or leaves in a suitable solvent. The solvent penetrates the plant material, dissolves the target compounds, and then the solvent - containing extract is separated from the solid residue. Common solvents used for Bilberry Extraction include ethanol, methanol, and water - based solvents. Ethanol is often preferred due to its relatively good solubility for the bioactive compounds, low toxicity, and its acceptability in the food and pharmaceutical industries.

2.2 Procedure

  1. First, the bilberry material is dried and ground to increase the surface area available for extraction.
  2. Then, a known amount of the solvent is added to the ground bilberry in a suitable extraction vessel. The ratio of solvent to bilberry material is an important parameter that can affect the extraction efficiency.
  3. The mixture is stirred or agitated at a specific temperature and for a certain period of time. For example, extraction may be carried out at room temperature for several hours or at an elevated temperature (e.g., 40 - 60°C) for a shorter time to enhance the solubility of the compounds.
  4. After extraction, the mixture is filtered to separate the liquid extract from the solid residue. The filtrate may be further concentrated under reduced pressure to obtain a more concentrated Bilberry Extract.

2.3 Pros

  • High Yield: Solvent extraction can often achieve relatively high yields of bioactive compounds, especially when optimized extraction conditions are used. For example, with the appropriate choice of solvent, temperature, and extraction time, a significant amount of anthocyanins can be extracted from European bilberry.
  • Cost - Effective: Solvent extraction is a relatively inexpensive method compared to some other advanced extraction techniques. The solvents used, such as ethanol, are widely available and affordable, and the equipment required for the process is not overly complex or costly.
  • Flexibility: This method allows for flexibility in terms of solvent selection and extraction conditions. Different solvents or solvent mixtures can be used depending on the target compounds and the desired properties of the extract. For instance, a water - ethanol mixture can be adjusted to different ratios to optimize the extraction of both polar and non - polar compounds.

2.4 Cons

  • Solvent Residue: One of the major drawbacks of solvent extraction is the potential for solvent residue in the final extract. Even after evaporation and concentration steps, traces of the solvent may remain, which can be a concern in applications where high purity is required, such as in pharmaceutical formulations.
  • Environmental Impact: The use of organic solvents, especially in large - scale industrial processes, can have a negative environmental impact. Solvents need to be disposed of properly, and their production and use may contribute to air and water pollution if not managed well.
  • Selectivity: Solvent extraction may not be highly selective for certain bioactive compounds. Along with the desired compounds, other unwanted substances may also be extracted, which may require additional purification steps to obtain a high - quality extract.

3. Supercritical Fluid Extraction

3.1 Principles

Supercritical fluid extraction (SFE) is a more advanced extraction technique that utilizes supercritical fluids as the extraction medium. A supercritical fluid is a substance that is above its critical temperature and critical pressure, where it exhibits properties intermediate between those of a gas and a liquid. For Bilberry Extraction, carbon dioxide (CO₂) is the most commonly used supercritical fluid due to its relatively low critical temperature (31.1°C) and critical pressure (73.8 bar), non - toxicity, non - flammability, and availability in high purity. In the SFE process, the supercritical CO₂ penetrates the bilberry matrix, selectively dissolves the target bioactive compounds, and then the extract is separated from the supercritical fluid by reducing the pressure or changing the temperature.

3.2 Procedure

  1. The bilberry material is first prepared by drying and grinding as in solvent extraction.
  2. The ground bilberry is placed in the extraction vessel of the SFE equipment. Supercritical CO₂ is then introduced into the vessel at the appropriate pressure and temperature to reach the supercritical state.
  3. The extraction is carried out for a specific period of time, during which the supercritical CO₂ continuously extracts the bioactive compounds from the bilberry.
  4. After extraction, the pressure is reduced, causing the supercritical CO₂ to return to the gaseous state and the extract to be collected separately.

3.3 Pros

  • High Purity and Selectivity: SFE offers high selectivity for specific bioactive compounds. Since the solubility of different compounds in supercritical CO₂ can be controlled by adjusting the pressure and temperature, it is possible to selectively extract the desired compounds from European bilberry while leaving behind unwanted substances. This results in a higher - quality extract with a higher purity of the target compounds.
  • No Solvent Residue: As supercritical CO₂ is a gas under normal conditions, there is no solvent residue in the final extract. This is a significant advantage, especially for applications in the food and pharmaceutical industries where purity is crucial.
  • Environmentally Friendly: CO₂ is a natural and non - toxic gas. The use of supercritical CO₂ in extraction has a much lower environmental impact compared to organic solvents. Moreover, CO₂ can be recycled and reused in the SFE process, further reducing the environmental footprint.

3.4 Cons

  • High Equipment Cost: The SFE equipment is relatively expensive compared to that used in solvent extraction. It requires high - pressure vessels, pumps, and precise control systems for temperature and pressure, which can be a significant investment, especially for small - scale operations.
  • Lower Yield in Some Cases: Although SFE can be highly selective, in some cases, the overall yield of bioactive compounds may be lower compared to solvent extraction. This may be due to the relatively lower solubility of some compounds in supercritical CO₂ under certain extraction conditions.
  • Complex Operation: The SFE process is more complex and requires more technical expertise to operate. The precise control of pressure, temperature, and extraction time is crucial for obtaining a good extraction result, and any deviation from the optimal conditions can affect the yield and quality of the extract.

4. Comparison and Best Method Selection

4.1 Yield Comparison

Solvent extraction can often achieve high yields, especially when optimized. However, supercritical fluid extraction may have a lower yield in some cases, particularly for compounds with relatively low solubility in supercritical CO₂. For maximizing the yield of a wide range of bioactive compounds from European bilberry, solvent extraction may be a better choice in some situations. But if the focus is on obtaining a specific high - value compound with high purity, the selectivity of SFE may compensate for the potentially lower overall yield.

4.2 Quality Comparison

Supercritical fluid extraction offers a higher quality extract in terms of purity and selectivity. The absence of solvent residue and the ability to selectively extract target compounds make SFE - derived extracts more suitable for high - end applications such as pharmaceuticals. Solvent extraction, on the other hand, may require additional purification steps to remove solvent residues and unwanted substances, which can affect the quality of the final product.

4.3 Environmental Impact Comparison

Supercritical fluid extraction is clearly more environmentally friendly due to the use of non - toxic and recyclable CO₂ as the extraction medium. Solvent extraction, especially when using large amounts of organic solvents, has a more significant environmental footprint, requiring proper solvent disposal and management to minimize pollution.

4.4 Best Method Selection

The choice of the best extraction method depends on various factors. For large - scale industrial production where cost - effectiveness and high yield are of primary importance, solvent extraction may be a more viable option, provided that proper measures are taken to address solvent residue and environmental issues. For applications where high purity, selectivity, and environmental friendliness are crucial, such as in the production of high - quality nutraceuticals and pharmaceuticals, supercritical fluid extraction is the preferred method. In some cases, a combination of both methods may also be considered, where solvent extraction is used first to obtain a relatively high - yield crude extract, followed by supercritical fluid extraction for further purification and isolation of specific compounds.

5. Conclusion

Both solvent extraction and supercritical fluid extraction have their own advantages and disadvantages for extracting European bilberry extract. Solvent extraction is a cost - effective and flexible method with the potential for high yields, but it has issues related to solvent residue and environmental impact. Supercritical fluid extraction offers high purity, selectivity, and environmental friendliness, but has higher equipment costs and may have lower yields in some cases. The best method should be selected based on the specific requirements of the end - product, production scale, and economic and environmental considerations.



FAQ:

What is European bilberry?

European bilberry (Vaccinium myrtillus) is a small, dark - blue berry - bearing shrub native to Europe. It has been used in traditional medicine for various purposes due to its rich content of bioactive compounds such as anthocyanins, flavonoids, and tannins.

What is solvent extraction for European bilberry extract?

Solvent extraction involves using a solvent (such as ethanol or methanol) to dissolve the bioactive compounds from the bilberry. The bilberry is usually ground and then soaked in the solvent for a certain period. After that, the solvent with the dissolved compounds is separated from the solid residue, and the solvent is then evaporated to obtain the extract. For example, ethanol is a common solvent as it can effectively extract anthocyanins while being relatively safe compared to some other solvents.

What are the advantages of solvent extraction?

One advantage is its relatively high yield. It can extract a significant amount of bioactive compounds from the bilberry. It is also a well - established method, which means it is easy to operate and does not require extremely specialized equipment. In addition, different solvents can be chosen according to the specific target compounds to be extracted.

What are the disadvantages of solvent extraction?

The main disadvantage is the potential for solvent residues in the final extract, which may pose safety risks if not removed completely. Also, some solvents can be flammable or toxic, requiring careful handling. From an environmental perspective, the use of large amounts of solvents may contribute to pollution if not properly disposed of.

What is supercritical fluid extraction?

Supercritical fluid extraction uses a supercritical fluid, most commonly carbon dioxide (CO₂) in the supercritical state. In this state, the fluid has properties between those of a gas and a liquid. It can penetrate the bilberry matrix easily and selectively extract the desired compounds. The supercritical CO₂ is then depressurized to separate the extract from the fluid.

What are the advantages of supercritical fluid extraction?

It is a cleaner" method as supercritical CO₂ is non - toxic, non - flammable, and environmentally friendly. It can produce high - quality extracts with high purity as it has good selectivity for different compounds. There is also no solvent residue problem, which is beneficial for applications in the food and pharmaceutical industries.

What are the disadvantages of supercritical fluid extraction?

The equipment for supercritical fluid extraction is relatively expensive, which may limit its widespread use. Also, the extraction process is more complex compared to solvent extraction and requires strict control of parameters such as pressure and temperature.

Related literature

  • "Extraction of Bioactive Compounds from European Bilberry: A Review of Modern Techniques"
  • "Optimization of Solvent Extraction for European Bilberry Anthocyanins"
  • "Supercritical Fluid Extraction of Valuable Compounds from Vaccinium myrtillus: Process and Quality Considerations"
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